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NET GUI. **Methods** {#j_jtim-2019-0014_s_004} =========== Guidelines of the Declaration of Helsinki were followed. All study participants provided informed consent and approval for the study was granted by the local ethics committee. **Study design** {#j_jtim-2019-0014_s_005} ================ A national study, including patients with AF undergoing rhythm or rate control therapy, was performed to determine the proportion of patients with valvular AF who meet ESC 2017/2016 guidelines. **Patient population** {#j_jtim-2019-0014_s_006} ====================== All patients with AF undergoing rate or rhythm control therapy (either oral or i.v. amiodarone, sotalol, or flecainide) were included in the study. Patients were divided into those who fulfilled guidelines recommendations for valvular AF and those who did not. **Definition of valvular AF** {#j_jtim-2019-0014_s_007} ============================= The following criteria were used to define valvular AF: patients with any previous diagnosis of rheumatic mitral, aortic, or tricuspid valve disease, or patients with any previous diagnosis of severe dysfunction of one of these valves, who had a history of rheumatic fever, heart surgery, or who were exposed to known valvulotoxic medication in the past. Patients with a history of congenital heart disease (such as ventricular septal defect or valvular defect) and those with any known abnormality of the atrioventricular conduction system (e.g., atrioventricular block) were excluded. Patients with paroxysmal AF or who presented with any documented episode of symptomatic atrial tachyarrhythmia (i.e., paroxysmal AF) were also excluded. **Blood collection and preparation** {#j_jtim-2019-0014_s_008} ==================================== Blood samples (10 mL) were drawn from each patient in an EDTA tube and stored at 4 °C prior to transportation to the laboratory. Serum samples were prepared immediately upon receipt in the laboratory. All samples were stored in a -80 °C freezer until assayed. **Chemical analysis** {#j_j



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